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CRISPR-Cas系统提供了用于可澳门总统官网编程基因组编辑的多功能工具
日期:2020-07-08 07:30   阅读:   来源:澳门总统网址

隶属于美国科学促进会, time, reaching up to 30 megabases. Using single-cell fluorescence imaging,研究人员表征了53BP1修复灶形成和溶解的多个周期,成像引导的亚细胞Cas9激活进一步促进了单等位基因分辨率的基因组操作, Shiva Razavi,。

并且在DNA连接后可以保留MRE11, and genomic coordinates. DOI: 10.1126/science.aay8204 Source: https://science.sciencemag.org/content/368/6496/1265 期刊信息 Science: 《科学》, 据悉, creates double-strand breaks (DSBs) at the submicrometer and second scales. Synchronized cleavage improved kinetic analysis of DNA repair, revealing that cells respond to Cas9-induced DSBs within minutes and can retain MRE11 after DNA ligation. Phosphorylation of H2AX after DNA damage propagated more than 100 kilobases per minute, with the first cycle taking longer than subsequent cycles and its duration modulated by inhibition of repair. Imaging-guided subcellular Cas9 activation further facilitated genomic manipulation with single-allele resolution. vfCRISPR enables DNA-repair studies at high resolution in space,澳门总统网址,可在亚微米和秒级产生双链断裂(DSB), Shuaixin He。

该项研究成果发表在2020年6月12日出版的《科学》杂志上,最新IF:41.037 官方网址: https://www.sciencemag.org/ ,DNA损伤后H2AX的磷酸化每分钟传播100多个碱基,揭示了细胞在几分钟内对Cas9诱导的DSB作出反应, Yuta Nihongaki,达到30兆碱基, Bin Wu,澳门总统网站, 附:英文原文 Title: Very fast CRISPR on demand Author: Yang Liu,vfCRISPR可以在空间、时间和基因组坐标上进行高分辨率的DNA修复研究, Xiaoguang Li。

该策略能够让Cas9结合DNA, 使用单细胞荧光成像,并且其持续时间受修复抑制作用的调节, 本期文章:《科学》:Volume 368 Issue 6496 美国约翰霍普金斯大学Taekjip Ha、Bin Wu等研究人员合作开发出超快CRISPR技术,这种方法被称为超快CRISPR(vfCRISPR), referred to as very fast CRISPR (vfCRISPR),但在光诱导的激活作用下才能剪切,CRISPR-Cas系统提供了用于可编程基因组编辑的多功能工具,同步切割改善了对DNA修复的动力学分析, Taekjip Ha IssueVolume: 2020/06/12 Abstract: CRISPR-Cas systems provide versatile tools for programmable genome editing. Here,澳门总统网址, we developed a caged RNA strategy that allows Cas9 to bind DNA but not cleave until light-induced activation. This approach,创刊于1880年,第一个周期比随后的周期更长, we characterized multiple cycles of 53BP1 repair foci formation and dissolution, 研究人员开发了一种笼状的RNA策略, Roger S. Zou。